Journal
HYPERTENSION
Volume 62, Issue 1, Pages 134-139Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/HYPERTENSIONAHA.113.01500
Keywords
endothelial cells; endothelium-derived hyperpolarizing factor; TRPV4-K(Ca)2; 3 association; vascular relaxation
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Funding
- Hong Kong Research Grant Committee [TBRS T13-706/11, CUHK479109, CUHK478710, CUHK478011]
- Fundamental Research Funds for the Central Universities [JUSRP51311A]
- Program for New Century Excellent Talents in University of the Ministry of Education of China [NCET-12-0880]
- China National Science Foundation [31171100, 81100185]
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The small conductance and intermediate conductance Ca2+-activated K+ channels are known to be involved in the endothelium-dependent hyperpolarization. Ca2+ entry into endothelial cells stimulates these channels, causing membrane hyperpolarization in endothelial cells and underlying smooth muscle cells. In the present study, with the use of coimmunoprecipitation and double immunolabeling methods, we demonstrated a physical interaction of transient receptor potential vanilloid 4 (TRPV4) with K(Ca)2.3 in rat mesenteric artery endothelial cells. Acetylcholine and 4-PDD mainly acted through TRPV4-K(Ca)2.3 pathway to induce smooth muscle hyperpolarization and vascular relaxation. K(Ca)3.1 was also involved in the process but at a much lesser degree than that of K(Ca)2.3. Stimulating TRPV4-K(Ca)2.3 signaling pathway also increased local blood flow in mesenteric beds and reduced systemic blood pressure in anesthetized rats. In streptozotocin-induced diabetic rats, the expression levels of TRPV4 and K(Ca)2.3 were reduced, which could be an underlying reason for the dysfunction of endothelium-dependent hyperpolarization in these animals. These results demonstrated an important physiological and pathological role of TRPV4-K(Ca)2.3 signaling pathway in vascular endothelial cells.
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