Journal
HYPERTENSION
Volume 59, Issue 2, Pages 256-+Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/HYPERTENSIONAHA.111.182170
Keywords
syncytial knots; syncytial aggregates; microparticles; sFlt1; soluble VEGFR1; preeclampsia
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Funding
- National Institutes of Health [RO3 HD055219-03, P01 HD030367]
- Harvard Diversity and Community Partnership Faculty Fellowship Award
- Fundacao Calouste Gulbenkian
- Fundacao Champalimaud
- Ministerio da Saude e Fundacao para a Ciencia e Tecnologia (Portugal)
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The cardinal manifestations of the pregnancy-specific disorder preeclampsia, new-onset hypertension, and proteinuria that resolve with placental delivery have been linked to an extracellular protein made by the placenta, soluble fms-like tyrosine kinase 1 (sFlt1), that injures the maternal vasculature. However, the mechanisms by which sFlt1, which is heavily matrix bound, gain access to the systemic circulation remain unclear. Here we report that the preeclamptic placenta's outermost layer, the syncytiotrophoblast, forms abundant knots that are enriched with sFlt1 protein. These syncytial knots easily detach from the syncytiotrophoblast, resulting in free, multinucleated aggregates (50-150 mu m diameter) that are loaded with sFlt1 protein and mRNA, are metabolically active, and are capable of de novo gene transcription and translation. At least 25% of the measurable sFlt1 in the third-trimester maternal plasma is bound to circulating placental microparticles. We conclude that detachment of syncytial knots from the placenta results in free, transcriptionally active syncytial aggregates that represent an autonomous source of sFlt1 delivery into the maternal circulation. The process of syncytial knot formation, shedding of syncytial aggregates, and appearance of placental microparticles in the maternal circulation appears to be greatly accelerated in preeclampsia and may contribute to the maternal vascular injury that characterizes this disorder. (Hypertension. 2012; 59: 256-264.). Online Data Supplement
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