Journal
HYPERTENSION
Volume 60, Issue 1, Pages 231-238Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/HYPERTENSIONAHA.111.187559
Keywords
preeclampsia/pregnancy; blood flow regulation; NO; hypoxia; placenta; endothelial NO synthase
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Funding
- Canadian Institutes of Health Research [MOP-12772, MGC-13299]
- Heart and Stroke Foundation of Ontario, an Ontario Graduate Scholarship
- Genesis Research Foundation
- Molly Towell Perinatal Research Foundation
- Canadian Institutes of Health Research
- Mount Sinai Hospital
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Preeclampsia is associated with impaired uteroplacental adaptations during pregnancy and abnormalities in the endothelial NO synthase (eNOS)-NO pathway, but whether eNOS deficiency plays a causal role is unknown. Thus, the objective of the current study was to determine the role of eNOS in the mother and/or conceptus in uteroplacental changes during pregnancy using eNOS knockout mice. We quantified uterine artery blood flow using microultrasound, visualized the uteroplacental vasculature using vascular corrosion casts, and used pimonidazole and hypoxia-inducible factor 1 alpha immunohistochemistry as markers of hypoxia in the placentas of eNOS knockout mice versus the background strain, C57Bl/6J (wild type). We found that increases in uteroplacental blood flow, uterine artery diameter, and spiral artery length were reduced, and markers of placental hypoxia in the junctional zone were elevated in late gestation in eNOS knockout mice. Both maternal and conceptus genotypes contributed to changes in uterine artery diameter and flow. Despite placental hypoxia, placental soluble fms-like tyrosine kinase 1 and tumor necrosis factor-alpha mRNA, and in maternal plasma, soluble fms-like tyrosine kinase 1 were not elevated in eNOS knockout mice. Thus, our results show that both eNOS in the mother and the conceptus contribute to uteroplacental vascular changes and increased uterine arterial blood flow in normal pregnancy. (Hypertension. 2012; 60:231-238.) circle Online Data Supplement
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