Journal
HYPERTENSION
Volume 52, Issue 1, Pages 143-149Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/HYPERTENSIONAHA.107.101667
Keywords
adventitia; fibroblasts; myofibroblasts; migration; neointima; restenosis; NADPH oxidase
Categories
Funding
- National Institutes of Health [HL55425, HL079207, HL28982]
- Fund for Henry Ford Hospital
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The vascular adventitia is emerging as an important modulator of vessel remodeling. Adventitial myofibroblasts migrate to the neointima after balloon angioplasty, contributing to restenosis. We postulated that angiotensin II (Ang II) enhances adventitial myofibroblast migration in vitro via reduced nicotinamide-adenine dinucleotide phosphate oxidase derived H2O2 and that Nox4-based oxidase promotes migration. Ang II increased myofibroblast migration in a concentration-dependent manner, with a peak increase of 1023 +/- 83%. Rat adventitial myofibroblasts were cotransfected with human Nox4 and human p22-phox plasmids or an empty vector. PCR showed an 8-fold increase in human Nox4 and human p22-phox plasmid expression. Using RT-PCR with primers specifically designed for rat reduced nicotinamide-adenine dinucleotide phosphate oxidases, endogenous Nox levels were determined. Ang II decreased endogenous Nox4 and Nox1 mRNA to 41% and 27% of control, respectively, but had no effect on Nox2. Cotransfection with human Nox4 and human p22-phox plasmids combined with Ang II reduced endogenous Nox4 mRNA levels (37 +/- 5% of control; P < 0.05), whereas it had no significant effect on Nox1 or Nox2. In empty vector-transfected cells, Ang II increased myofibroblast migration by 192 +/- 32% versus vehicle (P < 0.01) while increasing H2O2 (473 +/- 22% versus control; P < 0.001). Cotransfection with human Nox4 and human p22-phox plasmids decreased Ang II-induced migration (46 +/- 6%; P < 0.001) in parallel with attenuation of H2O2 production (23 +/- 8% versus empty vector; P < 0.05). Our data suggest that Nox4 promotes Ang II-induced myofibroblast migration via an H2O2-dependent pathway. The data also suggest that Nox4 causes feedback inhibition of its own expression in adventitial myofibroblasts. (Hypertension. 2008;52:143-149.)
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