4.7 Article

Isolation and characterization of Acidithiobacillus ferrooxidans strain QXS-1 capable of unusual ferrous iron and sulfur utilization

Journal

HYDROMETALLURGY
Volume 136, Issue -, Pages 51-57

Publisher

ELSEVIER SCIENCE BV
DOI: 10.1016/j.hydromet.2013.03.005

Keywords

Acidithiobacillus ferrooxidans; Subcellular structure; Marmatite bioleaching; Ferrous iron oxidation; Sulfur oxidation

Funding

  1. National Basic Research Program of P.R. China [2004CB619204, 2010CB630901]
  2. Program of New Century Excellent Talents in Ministry of Education of China [NCET-07-0869]
  3. National Natural Science Foundation of P.R. China [50321402, 50374075]

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A novel Acidithiobacillus ferrooxidans strain QXS-1 was isolated from the acid mine drainage of Qixiashan Pb-Zn-Ag mine area in China. The isolate QXS-1 is a motile, rod-shaped Gram-negative bacterium with an optimum growth at 30 degrees C and pH 2.0. The phylogenetic analysis has demonstrated that QXS-1 is a new strain of A. ferrooxidans. The strain QXS-1 exhibited an unusual trait on the utilization of ferrous iron and sulfur as energy sources. The sulfur-domesticated QXS-1 cells only utilized sulfur as energy source for growth when both ferrous iron and sulfur are available, whereas ferrous-domesticated QXS-1 cells prefer to simultaneously utilize both ferrous iron and sulfur as energy source for growth in the presence of both substrates. The QXS-1 cells accumulated intracellular hydrophilic sulfur during growth. Based on the ultrastructure study, we deduced that the accumulated sulfur in QXS-1 exists in both octasulfur ring form and polysulfide form. The strain QXS-1 exhibited remarkable marmatite-bioleaching capacity. It is able to extract 6585 mg/L zinc from marmatite within 18 days in shake flask. (C) 2013 Elsevier B.V. All rights reserved.

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