Journal
LETTERS IN APPLIED MICROBIOLOGY
Volume 60, Issue 3, Pages 298-306Publisher
WILEY-BLACKWELL
DOI: 10.1111/lam.12379
Keywords
degradation; odour remediation; pure culture; Rhodopseudomonas; skatole (3-methylindole: 3MI)
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Skatole (3MI) is a major contributor to the malodor emission resulting from ruminant and human faeces. The remediation of malodor has been a major challenge for the animal production industry. In this investigation, a pure culture of purple nonsulphur bacterium capable of degrading 3MI was isolated from a swine waste lagoon using an enrichment technique and identified as Rhodopseudomonas palustris WKU-KDNS3 based on 16S rRNA analysis and UV-visible spectroscopy. The cell structure of the organism was confirmed by transmission electron microscopy. Growth profile and 3MI removal pattern were determined using media supplemented with 01mol 3MI under short-term and long-term aerobic growth conditions. The organism grew on 3MI media as luxuriantly as control (without 3MI). Growth of R.palustris WKU-KDNS3 demonstrated a significant reduction in the level of 3MI (>48%) in 72h. The level of 3MI dropped further by >93% of the total concentration present in the medium in 21days. Skatole remediation potential of R.palustris WKU-KDNS3 can be judiciously utilized in various animal and industrial waste treatment systems.
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