Journal
HUMAN PATHOLOGY
Volume 42, Issue 3, Pages 361-368Publisher
W B SAUNDERS CO-ELSEVIER INC
DOI: 10.1016/j.humpath.2010.03.009
Keywords
GAS1; Stage II and III colorectal cancer; Cancer recurrence
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Funding
- Shanghai Science and Technology Committee, Shanghai, China [07dz19505]
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Growth arrest specific gene 1 had been associated with cell-cycle arrest, proliferation, and apoptosis. The aim of this study was to investigate the correlations between clinicopathologic factors and survival time and growth arrest specific gene 1 expression in patients with stage II and III colorectal cancer (CRC). Quantitative real-time polymerase chain reaction was performed in 64 fresh CRC tissues to examine growth arrest specific gene 1 mRNA expression. Six metastasis-derived and primary-derived cell lines were subjected to quantitative real-time polymerase chain reaction and Western blotting for further examination of both mRNA and protein concentrations. Growth arrest specific gene 1 protein was immunostained in 118 paraffin-embedded specimens. Growth arrest specific gene 1 expression was down-regulated both in tissues with recurrence and in metastasis-derived cell lines. Expression was unrelated to sex, age, tumor grade, or lymphovascular or perineural invasion. However, it was positively related to disease-free survival time (P < .05). Furthermore lower growth arrest specific gene 1 expression indicated a poorer survival rate (P < .05; log-rank test). Multivariate analysis also showed weak growth arrest specific gene 1 protein expression to be an independent adverse prognosticator (P < .05). Taken together, our results support the idea that growth arrest specific gene 1 contributes to predicting metastasis or recurrence in stage II and III CRC. (C) 2011 Elsevier Inc. All rights reserved.
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