Journal
HUMAN MUTATION
Volume 34, Issue 7, Pages 974-985Publisher
WILEY
DOI: 10.1002/humu.22327
Keywords
human; retrotransposon; transduction; polyadenylation; genome
Categories
Funding
- Wellcome Trust [075163/Z/04/Z]
- National Institutes of Health [T32GM7544, T32000040, GM060518]
- Wellcome Trust VIP Award
- BBSRC Doctoral Training Grant [BB/F016727/1]
- University of Michigan Cancer Center Support Grant [5P30CA4659]
- Howard Hughes Medical Institute
- BBSRC [BB/F016727/1] Funding Source: UKRI
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Long INterspersed Element-1 (LINE-1 or L1) retrotransposons are the only autonomously active transposable elements in the human genome. The average human genome contains approximate to 80-100 active L1s, but only a subset of these L1s are highly active or hot'. Human L1s are closely related in sequence, making it difficult to decipher progenitor/offspring relationships using traditional phylogenetic methods. However, L1 mRNAs can sometimes bypass their own polyadenylation signal and instead utilize fortuitous polyadenylation signals in 3 flanking genomic DNA. Retrotransposition of the resultant mRNAs then results in lineage specific sequence tags (i.e., 3 transductions) that mark the descendants of active L1 progenitors. Here, we developed a method (Transduction-Specific Amplification Typing of L1 Active Subfamilies or TS-ATLAS) that exploits L1 3 transductions to identify active L1 lineages in a genome-wide context. TS-ATLAS enabled the characterization of a putative active progenitor of one L1 lineage that includes the disease causing L1 insertion L1RP, and the identification of new retrotransposition events within two other hot L1 lineages. Intriguingly, the analysis of the newly discovered transduction lineage members suggests that L1 polyadenylation, even within a lineage, is highly stochastic. Thus, TS-ATLAS provides a new tool to explore the dynamics of L1 lineage evolution and retrotransposon biology.
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