Journal
HUMAN MOLECULAR GENETICS
Volume 23, Issue 21, Pages 5720-5732Publisher
OXFORD UNIV PRESS
DOI: 10.1093/hmg/ddu287
Keywords
-
Funding
- Duchenne Parent Project Netherland (DPP/NL)
- Italian MIUR-PRIN project [20108YB5W3_004]
Ask authors/readers for more resources
Weakness and fatigability are typical features of Duchenne muscular dystrophy patients and are aggravated in dystrophic mdx mice by chronic treadmill exercise. Mechanical activity modulates gene expression and muscle plasticity. Here, we investigated the outcome of 4 (T4, 8 weeks of age) and 12 (T12, 16 weeks of age) weeks of either exercise or cage-based activity on a large set of genes in the gastrocnemius muscle of mdx and wild-type (WT) mice using quantitative real-time PCR. Basal expression of the exercise-sensitive genes peroxisome-proliferator receptor gamma coactivator 1 alpha (Pgc-1 alpha) and Sirtuin1 (Sirt1) was higher in mdx versus WT mice at both ages. Exercise increased Pgc-1 alpha expression in WT mice; Pgc-1 alpha was downregulated by T12 exercise in mdx muscles, along with Sirt1, Ppar gamma and the autophagy marker Bnip3. Sixteen weeks old mdx mice showed a basal overexpression of the slow Mhc1 isoform and Serca2; T12 exercise fully contrasted this basal adaptation as well as the high expression of follistatin and myogenin. Conversely, T12 exercise was ineffective in WT mice. Damage-related genes such as gp91-phox (NADPH-oxidase2), Tgf beta, Tnf alpha and c-Src tyrosine kinase were over-expressed in mdx muscles and not affected by exercise. Likewise, the anti-inflammatory adiponectin was lower in T12-exercised mdx muscles. Chronic exercise with minor adaptive effects in WT muscles leads to mal-adaptation in mdx muscles with a disequilibrium between protective and damaging signals. Increased understanding of the pathways involved in the altered mechanical-metabolic coupling may help guide appropriate physical therapies while better addressing pharmacological interventions in translational research.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available