Journal
HUMAN IMMUNOLOGY
Volume 75, Issue 8, Pages 923-929Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.humimm.2014.06.021
Keywords
Retinoic acid; IgA; Class switch; RAR alpha; B cell
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Funding
- National Research Foundation of Korea (NRF) - Korea Government (MEST) [2013R1A1A2008536, 2011-0014492]
- National Research Foundation of Korea [2011-0014492, 2013R1A1A2008536] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Retinoic acid (RA) is known to have several functions that lead to a potent mucosal IgA response. Nevertheless, its exact role in human IgA synthesis has yet to be elucidated. Thus, we investigated the role of RA in promoting IgA isotype switching in human B cells. We found that RA increased IgA production and the expression of germ-line IgA1 and IgA2 transcripts (GLT alpha 1 and GLT alpha 2). This induction occurred alongside an increase in the frequency of IgA1-secreting B cell clones, as assessed by limiting dilution analysis. Under the same conditions, RA did not increase IgM and IgG production. Am80, an agonist of RA receptor alpha (RAR alpha), increased IgA production. In addition, RA activity was abrogated by LE540, an antagonist of RAR, suggesting that the RAR pathway is involved in RA-induced IgA production. Taken together, these results indicate that RA induces IgA isotype switching mainly through RAR alpha in human B cells. (C) 2014 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.
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