4.2 Article

Hypermethylation and low transcription of TLR2 gene in chronic periodontitis

Journal

HUMAN IMMUNOLOGY
Volume 74, Issue 9, Pages 1231-1236

Publisher

ELSEVIER SCIENCE INC
DOI: 10.1016/j.humimm.2013.04.037

Keywords

-

Categories

Funding

  1. Fundacao de Amparo Pesquisa de Minas Gerais
  2. Pro-reitoria de Pesquisa da Universidade Federal de Minas Gerais (Programa Institucional de Auxilio a Pesquisa de Doutores Recem-Contratados da PRPq/UFMG) (Belo Horizonte, Minas Gerais, Brazil)

Ask authors/readers for more resources

Periodontitis is an inflammatory disorder characterized by interactions between periodontal pathogens and host's immune response. Epigenetic may contribute to disease development and outcome by influencing the expression of genes involved in the immune response. It has been shown that Toll-like receptors (TLR) play an important role in the response to periodontopathic bacteria. The aim of study was to evaluate the methylation status and the expression of TLR2 gene in gingival samples from individuals with and without periodontitis. DNA was analyzed using the Methyl Profiler DNA Methylation qPCR assay. DNA methylation and transcript levels were evaluated by real-time polymerase chain reaction. The periodontitis group showed a hypermethylated profile and a low expression of gene. Positive correlation between the TLR2 methylation frequency and probing depth was observed. This study gives the first evidence of methylation frequency in inflamed periodontal tissues and of the possible participation of methylation in the development of periodontitis. (C) 2013 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.2
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available