Journal
HUMAN IMMUNOLOGY
Volume 70, Issue 7, Pages 496-501Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.humimm.2009.04.001
Keywords
Luminex (R) assay; Antibody testing; Nonspecific reactivity; Crossmatch testing
Categories
Ask authors/readers for more resources
Substances occurring naturally in the sera of patients can interfere with Luminex (R) antibody assays, causing increased background and changes in antibody specificity. We present data on the effectiveness of hypotonic dialysis (HD) or dithiothreitol (DTT) treatment in eliminating this interference. HD significantly increased reaction strength of positive control beads and reduced reaction strength of negative control beads. HID also improved specificity identification, determination of donor-specific antibody (DSA) strength, and crossmatch predictability compared with values in untreated serum. DTT also increased the reaction strength of positive control beads, but in most cases, further increased reactivity of negative control beads. DTT improved crossmatch predictability but to a lesser extent than did HD and may differ with specificities defined in other assays. Because interference is frequently observed in sera from highly sensitized patients, it is important to recognize and eliminate interference in Luminex (R) antibody assays for accurate and meaningful test interpretation. (C) 2009 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available