Journal
HUMAN GENE THERAPY
Volume 20, Issue 9, Pages 930-942Publisher
MARY ANN LIEBERT, INC
DOI: 10.1089/hum.2009.060
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Funding
- NIDDK [P30 DK47757]
- NHLBI [P01 HL59407]
- NICHD [P01 HD57247]
- GlaxoSmithKline
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Gene transfer to murine liver with vectors based on novel adeno-associated virus (AAV) serotypes is efficient, stable, and safe even in the setting of antigenic transgene products. We undertook a study in cynomolgus macaques to evaluate the relevance of these findings to primates. The vectors were based on AAV serotype 7 and expressed green fluorescence protein (GFP) from the cytomegalovirus enhanced beta-actin promoter in both single-stranded and self-complementary genomes. Transduction efficiencies from the single-stranded vectors were similar to those observed in mice, although there was no advantage in primates with the self-complementary vectors. Primates elicited vibrant cytotoxic T cell responses to GFP that correlated with hepatitis and loss of transgene expression. There was no evidence of T cell activation in response to the AAV capsid. These studies indicate that under some conditions primates may activate more robust T cell responses to transgene products than is observed in mice.
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