Journal
LANGMUIR
Volume 31, Issue 41, Pages 11305-11310Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.langmuir.5b03183
Keywords
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Funding
- Deutsche Forschungsgemeinschaft [TRR 61]
- NSFC-DFG [TRR 61]
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Single-molecule force spectroscopy measurements of a DNA duplex comprising an artificial metal-mediated base pair are reported. The measurements reveal that DNA duplexes comprising one central imidazole:imidazole mispair rupture at lower forces than a related duplex with canonical base pairs only. In contrast, DNA duplexes with one central imidazole-Ag+-imidazole base pair (formed by the addition of Ag+ to the aforementioned duplex with the mispair) rupture at higher forces. These measurements indicate for the first time that the increase in thermal stability of a nucleic acid duplex that is observed upon the formation of a metal-mediated base pair is accompanied by a concomitant mechanical stabilization. In fact, the mechanical stabilization even exceeds the thermal one. This result indicates that nucleic acids with metal-mediated base pairs should be ideal building blocks for rigid functionalized DNA nano-objects.
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