Journal
HISTOPATHOLOGY
Volume 59, Issue 2, Pages 283-291Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1365-2559.2011.03929.x
Keywords
carcinoma in situ; E-cadherin; oral epithelial dysplasia; two-phase appearance; beta-catenin
Categories
Funding
- Japan Society for the Promotion of Science
- Grants-in-Aid for Scientific Research [23792099, 22791810, 23406038, 20406029] Funding Source: KAKEN
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Aims: One of the important histopathological characteristics of oral epithelial dysplasia is a two-phase appearance of rete processes, comprising an upper layer of keratinized cells and a lower layer of basaloid cells, and thereby creating a sharp contrast between these two separate cell populations. The aim of this study was to determine the cellular adhesion status of the basaloid cells. Methods and results: Immunohistochemistry for beta-catenin, E-cadherin and their related molecules was carried out in surgical specimens of two-phase epithelial dysplasia of the oral mucosa. The lower-half basaloid cells and the upper keratinized cells were microdissected separately, and extracted DNA samples were subjected to methylation-specific polymerase chain reaction amplification for E-cadherin. beta-Catenin was immunolocalized within the nuclei and cytoplasm of Ki67-positive lower-half basaloid cells, as well as on the cell membrane of upper parakeratotic cells. The basaloid cells of the lower-half were also positive for matrix metalloproteinase-7 and cyclin D1, beta-catenin target gene products, alpha-dystroglycan, tenascin-C, and perlecan, but not for E-cadherin. The promoter region of the E-cadherin gene was hypermethylated. Conclusions: The solid proliferation of lower-half E-cadherin-free basaloid cells is enhanced by Wnt signalling cascades, as well as by the intraepithelial extracellular matrix or its bound growth factors.
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