Journal
HISTOCHEMISTRY AND CELL BIOLOGY
Volume 136, Issue 2, Pages 117-130Publisher
SPRINGER
DOI: 10.1007/s00418-011-0848-5
Keywords
Lectins; Histochemistry; Light microscopy; Electron microscopy; Colloidal gold; Digoxigenin; Silver amplification
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Funding
- Ministry of Education, Science and Technology [R31-2008-000-10086-0]
- National Research Foundation of Korea by the Ministry of Education, Science and Technology [2010-0027736]
- Swiss National Science Foundation
- National Research Foundation of Korea [R31-2011-000-10086-0] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Lectins have been proven to be invaluable reagents for the histochemical detection of glycans in cells and tissues by light and electron microscopy. This technical review deals with the conditions of tissue fixation and embedding for lectin labeling, as well as various markers and related labeling techniques. Furthermore, protocols for lectin labeling of sections from paraffin and resin-embedded tissues are detailed together with various controls to demonstrate the specificity of the labeling by lectins.
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