Journal
HISTOCHEMISTRY AND CELL BIOLOGY
Volume 134, Issue 1, Pages 75-82Publisher
SPRINGER
DOI: 10.1007/s00418-010-0708-8
Keywords
Folliculogenesis; Oocyte; Akt; Insulin
Categories
Funding
- National Basic Research Program of China (973 Program) [2007CB947401, 2006CB504003]
- National Nature Science Foundation [30700422]
- 863 Program of China [2008AA101003]
- Foundation of Taishan Scholar of Shandong Province
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Folliculogenesis is a critical process during mammalian development, but little is known about the mechanisms underlying primordial follicular formation, growth, and activation. The objective of this study was to assess the effect of continuous insulin exposure on folliculogenesis in 16.5 dpc and 3 dpp mouse ovaries in vitro. The results demonstrated that 5 mu g/ml insulin promotes the phosphorylation of Akt and the primordial follicular assembly and activation process via the InsR/PI3K/Akt signaling pathway in the 16.5 dpc fetal mouse ovaries cultured in vitro. However, the presence of 5 mu g/ml insulin represses the phosphorylation of Akt and the follicular assembly and activation process in the 3 dpp mouse ovaries cultured in vitro. Furthermore, in vitro cultures of 3 dpp mouse ovaries with insulin can repress the expression of Pten. In conclusion, folliculogenesis was stage-specifically regulated by insulin via the Akt signaling pathway in vitro.
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