Journal
HISTOCHEMISTRY AND CELL BIOLOGY
Volume 133, Issue 4, Pages 449-454Publisher
SPRINGER
DOI: 10.1007/s00418-010-0679-9
Keywords
Photodynamic therapy; Photosensitizer; Liposomes; Macropinocytosis; Clathrin-dependent endocytosis
Categories
Funding
- Ministerio de Ciencia e Innovacion Spain [CTQ2007-67763-C02/BQU]
Ask authors/readers for more resources
The study of uptake mechanisms of therapeutic drugs has a growing interest in biomedical research. In this work the cell uptake and phototoxicity of the photosensitizer Zn(II)-phthalocyanine (ZnPc) in dipalmitoyl-phosphatidyl-choline liposomes have been studied in the presence or absence of inhibitors of macropinocytosis (cytochalasin D), and clathrin-mediated endocytosis (dynasore). No differences in the uptake or photodynamic damage were observed in A-549 cells subjected to incubation with either ZnPc alone or in combination with cytochalasin D. On the contrary, co-incubation of A-549 cells with ZnPc and dynasore resulted in a significant decrease of photodamage as well as negligible uptake of the photosensitizer. These results indicate that ZnPc is internalized into cells preferentially by a mechanism of clathrin-mediated endocytosis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available