Journal
HISTOCHEMISTRY AND CELL BIOLOGY
Volume 131, Issue 1, Pages 115-127Publisher
SPRINGER
DOI: 10.1007/s00418-008-0503-y
Keywords
Hepatic stellate cells; Rho-GTP-ases; Myofibroblasts invasivity; GPI-anchored proteins; Lipid raft
Categories
Funding
- Deutsche Forschungsgemeinschaft [SFB 402, C6, D3, D4]
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Thy-1 (CD90) is an adhesion molecule induced in fibroblast populations associated with wound healing and fibrosis. In this study the question whether Thy-1-gene-expression can be induced in hepatic stellate cells (HSC) in vivo, under conditions of liver injury or liver regeneration was addressed. Acute and chronic rat liver injury was induced by the administration of CCl4. For comparison, cirrhotic human liver, and rat 67% partial hepatectomy (PH) was studied as well. Thy-1-gene-expression was examined also in isolated human liver myofibroblasts. Thy-1-mRNA expression was significantly upregulated in chronic liver injury. Thy-1(+) cells were detected in the periportal area of rat liver specimens in normal-, injured- and regenerative-conditions. In chronic human and rat liver injury, Thy-1(+) cells were located predominantly in scar tissue. In the pericentral necrotic zone after CCl4-treatment, no induction of Thy-1 was found. Gremlin and Thy-1 showed comparable localization in the periportal areas. Thy-1 was not detected in either normal or capillarized sinusoids, in isolated rat HSC, and was neither inducible by inflammatory cytokines in isolated HSC, nor upregulated in treated myofibroblasts. Based upon these data Thy-1 is not a marker of activated sinusoidal HSC, but it is a marker of activated (myo)fibroblasts found in portal areas and in scar tissue.
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