In vivo and in vitro effects of SREBP-1 on diabetic renal tubular lipid accumulation and RNAi-mediated gene silencing study

Lipid deposits can injury the kidney of diabetic patients and models. Sterol regulatory element binding protein-1 (SREBP-1) is transcription factor regulating the synthesis of fatty acid and triglyceride. At present whether the expression of SREBP-1 makes some effects on the lipid accumulation in diabetic kidney is not still clear completely. The purpose of our in vivo and in vitro study is to investigate the relationship between the expression of SREBP-1 and lipid abnormal metabolism in the type 1 diabetic rats and explore to inhibit SREBP-1 gene expression by RNA interfere in human renal proximal tubular epithelial cells line (HKC cells). The animal experiment showed that triglyceride and SREBP-1 were up-regulated in proximal tubule of diabetic rats' kidney, which may result in increase of transforming growth factor-beta 1 (TGF-beta 1) and accumulation of extracellular matrix (ECM). The further HKC cells experiment confirmed SREBP-1 increasing resulted into lipid droplet formation. The expression of fatty acid synthase (FAS) in HKC cells transfected with specific plasmid for SREBP-1 gene was significantly more than that of the cells transfected with the control plasmid pcDNA3.1 and that of the untransfected cells. Simultaneously, up-regulation of TGF-beta 1 and fibronectin, an ECM glycoprotein, was evident in HKC cells transfected by specific SREBP-1 plasmid. Furthermore, we found that high glucose was a positive factor on the expression of SREBP-1 at protein and mRNA levels in HKC cells. High glucose makes effects on SREBP-1 in time-dependent manner, and the greatest effect was at 48 h. In addition, two effective eukaryotic expression plasmid vectors of shRNA aimed at SREBP-1 were designed and constructed successfully. Compared with the negative control plasmid group, the levels of the expression of SREBP-1 were inhibited by 24.11 and 36.15%, respectively, at mRNA level, 20.80 and 37.59%, respectively, at precursor segment of protein level, and 38.12 and 52.24%, respectively, at mature segment of protein level at 48 h after transfection. In vivo and in vitro study suggested that high glucose caused increasing SREBP-1 mRNA and protein in renal proximal tubule epithelial cells of type 1 diabetic rats. Increasing SREBP-1 plays an important role in the pathogenesis of renal lipid accumulation by up-regulation of FAS and ECM accumulation by inducing TGF-beta 1 expression. The application of vector-mediated RNAi could markedly inhibit the expression of SREBP-1 in HKC cells, which is a promising tool for future research into the mechanisms of renal lipid accumulation in vivo.