Journal
HISTOCHEMISTRY AND CELL BIOLOGY
Volume 131, Issue 4, Pages 509-519Publisher
SPRINGER
DOI: 10.1007/s00418-008-0549-x
Keywords
Amyotrophic lateral sclerosis; Neurodegeneration; Neurotrophic factors; FGF-2; In situ hybridization histochemistry
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Impaired trophic support of motor neurons appears to be an important pathogenic factor in amyotrophic lateral sclerosis (ALS). We investigated the mRNA expression of the pluripotent fibroblast growth factor 2 (FGF-2) and its receptors in post mortem spinal cord of ALS and control patients. FGF-2 and FGF receptor (FGFR) 1 and 2 transcripts were first studied in the spinal cord using RT-PCR. The cellular distribution of FGF-2 and FGFR mRNA in the spinal cord, motor cortex and brain stem was then assessed by in situ hybridization histochemistry. RT-PCR revealed the presence of FGF-2 and FGF receptor 1 and 2 transcripts with no obvious differences between ALS and control spinal cord. Comparing mRNA expression in the motoneuron-containing ventral horn with the clinically and neuropathologically spared dorsal horn of ALS spinal cord displayed similar expression levels. At the cellular level, we found a prominent neuronal expression of FGF-2 and FGFR1. Interestingly, both morphologically intact and damaged motoneurons showed positive staining for FGF-2 and FGFR1 transcripts. The distribution of cells expressing FGF-2 and FGFR1 transcripts showed no differences between ALS and controls. Our data suggest that FGF-2 and FGFR1 expression is preserved in the motor system in end stage ALS.
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