Journal
HEREDITY
Volume 105, Issue 5, Pages 443-448Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/hdy.2010.51
Keywords
sex determination; sex reversal; sex chromosome; gonad; medaka fish
Categories
Funding
- Ministry of the Environment of Japan [ExTEND2005]
- Ministry of Education, Culture, Sport, Science and Technology of Japan [17052007, 19040009]
- Grants-in-Aid for Scientific Research [17052007, 19040009] Funding Source: KAKEN
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The teleost fish, medaka (Oryzias latipes), has an XX/XY sex-determining mechanism. A Y-linked DM domain gene, DMY, has been isolated by positional cloning as the sex-determining gene in this species. Previously, we conducted a field survey of genotypic sex and found that approximately 1% of wild medaka are sex-reversed (XX males and XY females). Here, we performed genetic analyses of nine spontaneous XX sex-reversed males to elucidate its genetic basis. In all cases, the F(1) progeny were all females, whereas XX males reappeared in the backcross (BC) progeny, suggesting that XX sex reversal is a recessive trait. Although the incidences of sex reversal in the BC progeny were mostly low, 40% were males derived from one XX male. We performed linkage analysis using 55 BC males and located a single major factor, sda-1 (sex-determining autosomal factor-1), controlling sex reversal in an autosomal linkage group. Thus, genes involved in the sex-determining pathway can be isolated from spontaneous mutants in wild populations. Heredity (2010) 105, 443-448; doi:10.1038/hdy.2010.51; published online 28 April 2010
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