Journal
HEPATO-GASTROENTEROLOGY
Volume 58, Issue 112, Pages 2081-2086Publisher
H G E UPDATE MEDICAL PUBLISHING S A
DOI: 10.5754/hge11220
Keywords
Bone marrow mesenchymal stem cells; Hepatocyte growth factor; Beta-nerve growth factor; Differentiation; Hepatocyte
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Funding
- Qingdao science and technology support plan projects [09-1-1-25-nsh]
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Background/Aims: To investigate the mechanism and regulation of differentiation from bone marrow mesenchymal stern cells (BMSCs) into hepatocytes and to find a new source for therapies of hepatic diseases. Methodology: We isolated BMSCs for subsequent differentiation in the presence of hepatocyte growth factor (HGF) or beta-nerve growth factor (beta-NGF). Cell morphology was observed and cell surface phenotypings were detected by flow cytometry. al-antitrypsin (AAT) expression of the hepatocytes was confirmed by immunocytochemistry and albumin expression was validated by real time PCR and western blotting. The expression of high-affinity nerve growth factor receptor (TrkA) and the activation of Erk pathway were detected by western blotting. Hepatocyte functional activity was confirmed by uptake of indocyanine green (ICG) assay. Results: Small round cells appeared in the presence of FIGF on day 10 or beta-NGF on day 12. Differentiated cells expressed albumin and had functional characteristics of hepatocytes, such as uptake of ICG. BMSCs were positive for TrkA. HGF and beta-NGF significantly upregulated the protein levels of phospho-Erk. Conclusions: BMSCs could differentiate into hepatocytes in the differentiation media including HGF or beta-NGF. Combination of HGF and beta-NGF significantly increased the efficiency of hepatic differentiation.
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