Journal
HEMATOLOGY
Volume 16, Issue 1, Pages 43-49Publisher
MANEY PUBLISHING
DOI: 10.1179/102453311X12902908411634
Keywords
T-cells; TRVB repertoire; Clonal expansion; Chronic myeloid leukemia
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Funding
- National '863' project [2006AA02Z114]
- National Natural Science Foundation of China [39870358, 30270579]
- Natural Science Foundation of Guangdong Province [05103293, 9251063201000001]
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T-cell immunodeficiency is a common feature in cancer patients, which may relate to initiation and development of tumor. In expanding our previous observations in this area, we studied the repertoire of T-cell receptor beta variable region (TRBV) and T-cell proliferative history in CD4(+) and CD8(+) T cells from chronic myeloid leukemia (CML) patients. The expression and clonality analysis were performed by reverse transcription-polymerase chain reaction (RT-PCR) and GeneScan technique in peripheral blood mononuclear cells (PBMCs), CD4(+) and CD8(+) subsets of T cells. Nineteen CML cases in chronic phase were selected for this study and 17 healthy individuals served as controls. Marked restriction of TRBV repertoire was observed in both CD4(+) and CD8(+) T cells from CML. In most CML samples, clonally expanded T cells were identified in CD4(+) and CD8(+) T cells, predominantly in TRBV19 and TRBV21 (5/19) subfamilies. In conclusion, the restricted expression of TRBV subfamilies indicates the T-cell immunodeficiency in CML patients; however, clonally expanded T cells suggest a specific immune response to leukemia associated antigens.
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