Journal
LAB ON A CHIP
Volume 15, Issue 20, Pages 3984-3988Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5lc00698h
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Funding
- Brain Korea 21 Plus Project [F14SN02D1310]
- National Research Foundation - Ministry of Education [NRF-2015R1A2A1A09005662]
- Ministry of Food and Drug Safety [15182MFDS455]
- Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea [HI14C14000]
- Agricultural Robotics and Automation Research Center through Agriculture, Food and Rural Affairs Research Center Support Program, Ministry of Agriculture, Food and Rural Affairs [714002-7]
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Current in vitro systems mimicking bone tissues fail to fully integrate the three-dimensional (3D) microvasculature and bone tissue microenvironments, decreasing their similarity to in vivo conditions. Here, we propose 3D microvascular networks in a hydroxyapatite (HA)-incorporated extracellular matrix (ECM) for designing and manipulating a vascularized bone tissue model in a microfluidic device. Incorporation of HA of various concentrations resulted in ECM with varying mechanical properties. Sprouting angiogenesis was affected by mechanically modulated HA-extracellular matrix interactions, generating a model of vascularized bone microenvironment. Using this platform, we observed that hydroxyapatite enhanced angiogenic properties such as sprout length, sprouting speed, sprout number, and lumen diameter. This new platform integrates fibrin ECM with the synthetic bone mineral HA to provide in vivo-like microenvironments for bone vessel sprouting.
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