Journal
LAB ON A CHIP
Volume 15, Issue 21, Pages 4138-4147Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5lc01000d
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Funding
- European Union FP7 grant 'Body on a chip' [ICT-FET-296257]
- ERC Advanced Grant NeuroCMOS [AdG 267351]
- Swiss National Science Foundation [142440]
- Marie Curie Research Training Network EngCaBra [264417]
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Microfluidic hanging-drop networks enable culturing and analysis of 3D microtissue spheroids derived from different cell types under controlled perfusion and investigating inter-tissue communication in multi-tissue formats. In this paper we introduce a compact on-chip pumping approach for flow control in hanging-drop networks. The pump includes one pneumatic chamber located directly above one of the hanging drops and uses the surface tension at the liquid-air-interface for flow actuation. Control of the pneumatic protocol provides a wide range of unidirectional pulsatile and continuous flow profiles. With the proposed concept several independent hanging-drop networks can be operated in parallel with only one single pneumatic actuation line at high fidelity. Closed-loop medium circulation between different organ models for multi-tissue formats and multiple simultaneous assays in parallel are possible. Finally, we implemented a real-time feedback control-loop of the pump actuation based on the beating of a human iPS-derived cardiac microtissue cultured in the same system. This configuration allows for simulating physiological effects on the heart and their impact on flow circulation between the organ models on chip.
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