Journal
LAB ON A CHIP
Volume 15, Issue 11, Pages 2412-2418Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5lc00159e
Keywords
-
Categories
Funding
- NIH-National Institute of Biomedical Imaging and Bioengineering [EB012597, EB017876]
- National Science Foundation [CMMI-1332681, CMMI-1120795]
- Saving tiny Heart Society
- California Institute of Regenerative Medicine
- ARRA grant from NIH-National Institute of Biomedical Imaging and Bioengineering [RC1 EB011780]
- NIH [DP020D006460]
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL095780] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [RC1EB011780, R01EB012597, R21EB017876] Funding Source: NIH RePORTER
Ask authors/readers for more resources
Long-term culture and monitoring of individual multicellular spheroids and embryoid bodies (EBs) remains a challenge for in vitro cell propagation. Here, we used a continuous 3D projection printing approach with an important modification of nonlinear exposure - to generate concave hydrogel microstructures that permit spheroid growth and long-term maintenance, without the need for spheroid transfer. Breast cancer spheroids grown to 10 d in the concave structures showed hypoxic cores and signs of necrosis using immunofluorescent and histochemical staining, key features of the tumor microenvironment in vivo. EBs consisting of induced pluripotent stem cells (iPSCs) grown on the hydrogels demonstrated narrow size distribution and undifferentiated markers at 3 d, followed by signs of differentiation by the presence of cavities and staining of the three germ layers at 10 d. These findings demonstrate a new method for long-term (e.g. beyond spheroid formation at day 2, and with media exchange) 3D cell culture that should be able to assist in cancer spheroid studies as well as embryogenesis and patient-derived disease modeling with iPSC EBs.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available