Journal
LAB ON A CHIP
Volume 15, Issue 6, Pages 1417-1423Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4lc01465k
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Funding
- California Sea Grant College Program (CASG)
- Stanford Woods Institute for the Environment
- Stanford Nano Shared Facilities Bio/Medical Mini Seed Grant
- Stanford Center for Innovation in Global Health
- 3M Non-tenured Faculty Award
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This paper describes an optofluidic droplet interrogation device capable of counting fluorescent drops at a throughput of 254000 drops per second. To our knowledge, this rate is the highest interrogation rate published thus far. Our device consists of 16 parallel microfluidic channels bonded directly to a filter-coated two-dimensional Complementary Metal-Oxide-Semiconductor (CMOS) sensor array. Fluorescence signals emitted from the drops are collected by the sensor that forms the bottom of the channel. The proximity of the drops to the sensor facilitates efficient collection of fluorescence emission from the drops, and overcomes the trade-off between light collection efficiency and field of view in conventional microscopy. The interrogation rate of our device is currently limited by the acquisition speed of CMOS sensor, and is expected to increase further as high-speed sensors become increasingly available.
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