Journal
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
Volume 37, Issue 7, Pages 1014-1021Publisher
WILEY-BLACKWELL
DOI: 10.1002/hed.23713
Keywords
pleomorphic adenoma; salivary gland; matrix-assisted laser desorption ionization (MALDI) imaging mass spectrometry; digital histology
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Funding
- European Union 7th Framework Programme [305259]
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BackgroundDespite efforts in localization of key proteins using immunohistochemistry, the complex proteomic composition of pleomorphic adenomas has not yet been characterized. Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI imaging) allows label-free and spatially resolved detection of hundreds of proteins directly from tissue sections and of histomorphological regions by finding colocalized molecular signals. Spatial segmentation of MALDI imaging data is an algorithmic method for finding regions of similar proteomic composition as functionally similar regions. MethodsWe investigated 2 pleomorphic adenomas by applying spatial segmentation to the MALDI imaging data of tissue sections. ResultsThe spatial segmentation subdivided the tissue in a good accordance with the tissue histology. Numerous molecular signals colocalized with histologically defined tissue regions were found. ConclusionOur study highlights the cellular transdifferentiation within the pleomorphic adenoma. It could be shown that spatial segmentation of MALDI imaging data is a promising approach in the emerging field of digital histological analysis and characterization of tumors. (c) 2014 Wiley Periodicals, Inc. Head Neck 37: 1014-1021, 2015
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