Journal
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
Volume 35, Issue 6, Pages 800-808Publisher
WILEY-BLACKWELL
DOI: 10.1002/hed.23034
Keywords
human papillomavirus; viral load; integration; E6; E7 mRNA; head and neck squamous cell carcinoma
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Funding
- Japan Society for the Promotion of Science [KAKENHI 22791614]
- Ryukyu Asia-Pacific Medical Exchange Association
- Japan-China Medical Association
- [KAKENHI 21591615]
- Grants-in-Aid for Scientific Research [23592535, 22791614] Funding Source: KAKEN
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Background The purpose of this study was to determine prospectively both human papillomavirus (HPV) load and physical status in different types of head and neck squamous cell carcinoma (HNSCC). Methods HPV DNA, E6/E7 mRNA expression, viral load, and physical status of 184 patients with HNSCC were examined simultaneously by polymerase chain reaction (PCR)-based methods. Results The HPV genome was detected in 54 HNSCC samples (29.3%), particularly in tonsillar carcinomas (69.6%). Compared with nonoropharyngeal HNSCC, oropharyngeal carcinoma harbored a relatively higher viral load, especially in tonsillar carcinoma. Although integrated or mixed status was observed in 75.6% of HPV-16positive samples, E6/E7 mRNA transcripts were detected in only 27.5% of HPV DNA-positive cases. High HPV-16 load correlated significantly with E6/E7 mRNA expression. Conclusion E6/E7 mRNA expression in patients with HNSCC with low viral load remains low even in cases of integration to the host genome. Tonsillar carcinomas were significantly associated with HPV among various types of HNSCC. (c) 2012 Wiley Periodicals, Inc. Head Neck 35: 800-808, 2013
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