Journal
HARMFUL ALGAE
Volume 28, Issue -, Pages 64-70Publisher
ELSEVIER
DOI: 10.1016/j.hal.2013.05.012
Keywords
cob gene; cox1; Dinophysis species; D. acuminata complex; DSP
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Funding
- Spanish Ministry of Science and Innovation [CTM2009-12988-001, CTM2009-12988-0O2]
- Universidad Autonoma de Madrid (UAM)
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The identification of Dinophysis species with similar morphology but different toxic (Diarrhetic Shellfish Poisoning, DSP) potential is a crucial task in harmful algae monitoring programmes. The taxonomic assignment of Dinophysis species using molecular markers is a difficult task due to extremely low interspecific variability within their nuclear ribosomal genes and intergenic regions. Mitochondrial cox1 gene has been proposed as a better specific marker for Dinophysis species based on its higher resolution for two morphologically related species (Dinophysis acuminata and Dinophysis ovum) of the Dinophysis acuminata complex. In this study, the potential of two mitochondrial genes (mt cox1 and cob) to discriminate among six Dinophysis species (field isolates and cultures) associated with DSP events was explored. Neither mt coxl nor cob genes provided enough resolution for all species of Dinophysis. The cob gene showed very poor resolution and grouped all Dinophysis spp. in a common clade. In contrast, the cox1 phylogeny distinguished 5 clades in the Dinophysiales the acuminata complex, the caudata group, acuta + norvegica and Phalacromaspp. However, within the D. acuminata complex mtcoxl is so far the unique marker that differentiates D. acuminata from other species: isolates of D. ovum and Dinophysis sacculus had almost identical sequences (only four mismatches), but they were well separated from D. acuminata. D. acuminata and Dinophysis skagii (considered a life cycle stage of the former) showed identical cox1 sequences. Probes towards this gene can be useful in Mediterranean and Western Iberia sites where the co-occurrence of close morphotypes of D. acuminata and D. sacculus pose a problem for monitoring analyses. This is the first report on cultures of D. sacculus and its phylogenetic relation with other species of the D. acuminata complex. 2013 Elsevier B.V. All rights reserved.
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