Journal
HARMFUL ALGAE
Volume 13, Issue -, Pages 83-88Publisher
ELSEVIER
DOI: 10.1016/j.hal.2011.10.006
Keywords
Alexandrium tamarense; Algicidal activity assay; Algicidal protein; Phylogenetic tree; Purification
Categories
Funding
- National Natural Science Foundation of China [40930847, 31070442, 40876061]
- National High Technology Research and Development Program of China [2008AA09Z408]
- Program for Changjiang Scholars and Innovative Research Team in University [40821063]
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Interactions between bacteria and harmful algal bloom (HAB) species have been acknowledged as an important factor of regulating the population of these algae. In the study, two strains of algicidal bacteria, DHQ25 and DHY3, were screened out because of their probably secreting algicidal proteins against axenic Alexandrium tamarense. Molecular characterization classified them to the gamma-proteobacteria subclass and to the genus Vibrio and Pseudoalteromonas, respectively. After centrifugation and ultrafiltration, chromatography of the cultural supernatants of DHQ25 revealed 8 peaks by HPLC. SDS-PAGE and Native PAGE determination showed that peak 7 to be a monoband peak. Both xenic and axenic culture of A. tamarense were susceptible to the purified protein (short for P7 below) indicated by algicidal activity assay. Observation of algicidal process demonstrated that algal cells were lysed and cellular substances were released under visual fields of microscope. P7 proved to be a challenge controller of A. tamarense by the above characterizations of algicidal activity assaying and algicidal process. This is the first report of a protein algicidal to the toxic dinoflagellate A. tamarense. The findings increase our knowledge of bacterial-algal interactions and the role of bacteria during controlling HABs. (C) 2011 Elsevier B.V. All rights reserved.
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