Journal
HARMFUL ALGAE
Volume 8, Issue 6, Pages 839-842Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.hal.2009.03.004
Keywords
Identification; Karenia mikimotoi; Loop-mediated isothermal amplification (LAMP); Ribosomal DNA internal transcribed spacer (ITS)
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Funding
- science and technology commission of Shanghai municipality [062358101]
- national non-profit institutes (East China Sea Fisheries Research Institute) [2007M22]
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A loop-mediated isothermal amplification (LAMP) assay was developed to detect the genomic DNA of Karenia mikimotoi using a set of four specific primers based on a ribosomal DNA internal transcribed spacer (ITS). The sensitivity of this LAMP assay was 100-fold higher than regular PCR, and its specificity was validated using other algae as a comparison. Two visual detection approaches were feasible to interpret the positive or negative results. This technology may have the potential to aid in forecasting red-tides on the scene because of its high sensitivity, specificity and rapid detection. (C) 2009 Elsevier B.V. All rights reserved.
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