The Gut-Brain-Axis: A Positive Relationship between Gut Microbial Dysbiosis and Glioblastoma Brain Tumour

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Authors

Hafiz Muhammad Ishaq¹

1. Department of Pathobiology & Biomedical Sciences, MNS University of Agriculture Multan, Pakistan

Conference / event

PREEF, July 2024 (Virtual)

Poster summary

The glioblastoma brain tumour (GBM) is the most aggressive and treatment-resistant malignancy. However, the gut-brain axis is crucial for the growth and stimulation of the CNS. Fecal samples were collected from 15 healthy volunteers and 25 GBM patients. To delve into the microbial content, we employed PCR-DGGE targeting the V3 region of the 16S rRNA gene. qPCR was also conducted to quantify the crucial intestinal bacteria. High-throughput sequencing targeting the V3+V4 region of the 16S rRNA gene was performed. Delving into genera statistics, a substantial increase in the abundance of Parasutterella, Escherichia-Shigella, and Bacteroides in GBM group compared to the control group. Furthermore, species results revealed a significant increase of Bacteroides vulgatus and Escherichia coli in the GBM group. Thus, GBM patients have significant gut microbial dysbiosis. So, the current research opens up new strategies to comprehend the disease mechanisms, pathways, and treatment of GBM patients.

Keywords

GBM, DGGE, High-throughput sequencing, Gut Bacteria, Dysbiosis

Research areas

Microbiology

References

No data provided

Funding

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Supplemental files

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Additional information

Competing interests

No competing interests were disclosed.

Data availability statement

Data sharing not applicable to this poster as no datasets were generated or analyzed during the current study.

Creative Commons license

Copyright © 2024 Ishaq. This is an open access work distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.