Ubiquitination of bacterial surface proteins act as novel innate pathogen sensing strategy

Authors

Shruti Apte¹ , Smita Bhutda¹ , Sourav Ghosh¹ , Anirban Banerjee¹

1. Indian Institute of Technology Bombay

Conference / event

Innate Immunity in Host-pathogen Interaction, July 2022 (Virtual)

Poster summary

Ubiquitination plays a pivotal role in the surveillance of intracellular milieu and elimination of a variety of pathogens. Here, we identify bacterial ubiquitination substrates, surface-exposed proteins present on phylogenetically diverse bacteria including Streptococcus pneumoniae (SPN) and Salmonella enterica serovar Typhimurium (STm). By biochemical and intracellular survival study, we reveal a strategy utilized by the host to recognize such targets based on sensing of degron-like motifs. Such consensus degron-like motifs resemble intra-cytosolic PAMPs (Pathogen associated molecular patterns) to aid in the recognition of diverse microbes. Remarkably, by applying this approach, we could remodel and stimulate non-ubiquitin targets to be identified by host ligases. We also showcase a novel anti-microbial role of SCFFBXW7 complex supported by a regulatory host kinase, GSK3β in pathogenic elimination. In conclusion, our study suggests that the host exploits a universal set of rules for recognizing various pathogens to conserve cellular resources and boost anti-microbial immunity.

Keywords

Bacterial pathogenesis, Cellular Immunity, Host-Pathogen interaction, Ubiquitination

Research areas

Biological Sciences, Microbiology

References

No data provided

Funding

No data provided

Supplemental files

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Additional information

Competing interests

No competing interests were disclosed.

Data availability statement

The datasets generated during and / or analyzed during the current study are available from the corresponding author on reasonable request.

Creative Commons license

Copyright © 2023 Apte et al. This is an open access work distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.