Selective dengue virus filtration and absorption system from plasma circulation

Authors

Siwaporn Boonyasuppayakorn¹ , Sasiwimon Thonghong¹ , Saran Salakij² , Nattachai Srisawat³ , Numpon Insin⁴ , Pat Sinananpat²

1. Applied Medical Virology Research Unit, Faculty of Medicine, Chulalongkorn University
2. Department of Mechanical Engineering, Faculty of Engineering, Chulalongkorn University
3. Division of Nephrology, Department of Medicine, Faculty of Medicine, Chulalongkorn University
4. Department of Chemistry, Faculty of Science, Chulalongkorn University

Conference / event

2022 Japan Design, Idea and Invention Expo, July 2022 (Virtual)

Poster summary

Introduction: Dengue infection is a significant public health concern that no specific treatment is available. An extracorporeal plasmapheresis or plasma filtration is a treatment option for severe cases with complications. However, the commercial adsorption devices mainly contained size-exclusive porous beads to adsorb the plasma proteins non-selectively. Methods: We developed a 1:50 simulated circuit for dengue virus-specific adsorption using a flavivirus-specific (4G2) antibody entrapped into the alginate bead. Results: The reduction ratio of the viral titer after 3 hours of continuous run were 63.00 ± 1.21 %, and 93.97 ± 1.27 % measured by reverse transcription qPCR, and plaque titration, respectively. No specific adsorption was observed with Enterovirus A71 or Escherichia coli bacteria. Conclusion: This study is a proof-of-concept for the potential use of dengue virus-specific adsorption column in the 1:50 simulated circuit. The system could be applied to various clinical platforms by substituting target-specific antibodies.

Keywords

Dengue, Plasma filtration, Absorbtion column, Virus filtration

Research areas

Immunology, Bioengineering, Microbiology, Mechanical Engineering, Molecular and Cellular Biosciences

References

1. https://doi.org/10.1159/000524387

Funding

1. Ratchadapiseksompotch Endowment Fund

Supplemental files

1. research article   Download

Additional information

Competing interests

No competing interests were disclosed.

Data availability statement

Data sharing not applicable to this poster as no datasets were generated or analyzed during the current study.

Creative Commons license

Copyright © 2023 Boonyasuppayakorn et al. This is an open access work distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.