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Effects of Artificial Light Spectra on Anthocyanin Accumulation in Blueberry Callus Cultures
PUBLISHED September 27, 2022 (DOI: https://doi.org/10.54985/peeref.2209p1758898)
NOT PEER REVIEWED
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Authors
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Gamil Rayan Abou El-Dis1 , Khusnetdinova Landysh Zavdetovna2 , Akulov Anton Nikolaevich3 , Walla Mohamed Abdelmaksood Abdelazeez4 , Timofeeva Olga Arnoldovna2
- Desert Research Center, Cairo, Egypt
- Kazan Federal University, Kazan, Russia
- Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS
- Faculty of Science, Al-Azhar University, Cairo
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Conference / event
- International Annual Conference on Basic and Applied Science (IACBAS 2022) Towards "Advanced Science & Sustainable Development", March 2022 (Cairo, Egypt)
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Poster summary
- Blueberry is considered a health-promoting food with a high content of anthocyanins. The extraction of anthocyanins from callus has been used to solve some problems of anthocyanins production, being more effective for commercial application and high production of anthocyanins. One of the most important aims of our study is to enhance anthocyanin synthesis in callus cultures. The results showed that callus induction from the highbush blueberry V. corymbosum L. cv. Sunt Blue Giant depends on the type of explant and culture medium. Maximum callus induction was established on Woody Plant medium (WPM) containing 2.0 mg/L ɑ–naphthalene acetic acid (NAA) with 1.0 mg/L 6-benzylaminopurine/benzyl adenine (BAP) from all used explants and the maximum fresh weight (for leaf, 1.03; root, 1.0; and stem, 0.8 g/jar) was recorded. After four subcultures on the same medium composition, we studied the effect of different light types on the accumulation of anthocyanins in callus culture.
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Keywords
- Blueberry, Blue light, In vitro, Subculture, Red light
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Research areas
- Agriculture, Biochemistry, Biological Sciences
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References
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- Wu X, Beecher GR, Holden JM, Haytowitz DB, Gebhardt SE, Prior RL (2006) Concentrations of anthocyanins in common foods in the United States and estimation of normal consumption. Journal of agricultural and food chemistry 54(11):4069–4075. https://doi.org/10.1021/jf060300l
- Li C, Feng J, Huang W-Y, An X-T (2013) Composition of polyphenols and antioxidant activity of rabbiteye blueberry (Vaccinium ashei) in Nanjing. Journal of Agricultural and Food Chemistry 61(3):523–531. https://doi.org/10.1021/jf3046158
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- Debnath SC, Goyali JC (2020) In Vitro Propagation and Variation of Antioxidant Properties in Micropropagated Vaccinium Berry Plants—A Review. Molecules 25(4):788. https://doi.org/10.3390/molecules25040
- Migas P, Cisowski W, Dembińska-Migas W (2005) Isoprene derivatives from the leaves and callus cultures of Vaccinium corymbosum var. Bluecrop. Acta Pol Pharm 62: 45–51
- Migas P, Luczkiewicz M, Cisowski W (2006) The influence of auxins on the biosynthesis of isoprene derivatives in callus cultures of Vaccinium corymbosum var. bluecrop. Z Naturforsch C 61:565–570. https://doi.org/10.1515/znc-2006-7-816
- Simões C, Bizarri CHB, da Silva Cordeiro L, de Castro TC, Coutada LCM, da Silva AJR, Albarello N, Mansur E (2009) Anthocyanin production in callus cultures of Cleome rosea: modulation by culture conditions and characterization of pigments by means of HPLC-DAD/ESIMS. Plant Physiology and Biochemistry 47(10):895–903. https://doi.org/10.1016/j.plaphy.2009.06.005
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Funding
- No data provided
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Supplemental files
- No data provided
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Additional information
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- Competing interests
- No competing interests were disclosed.
- Data availability statement
- The datasets generated during and / or analyzed during the current study are available from the corresponding author on reasonable request.
- Creative Commons license
- Copyright © 2022 Abou El-Dis et al. This is an open access work distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Abou El-Dis, G., Zavdetovna, K., Nikolaevich, A., Abdelazeez, W., Arnoldovna, T. Effects of Artificial Light Spectra on Anthocyanin Accumulation in Blueberry Callus Cultures [not peer reviewed]. Peeref 2022 (poster).
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